Preparation of $sup 14$C-gossypol by incorporation of acetate-1-$sup 14$C and acetate-2-$sup 14$C by biosynthesis

Journal Article · Sun Sep 01 00:00:00 EDT 1974 · J. Am. Oil Chem. Soc., v. 5, no. 9, pp. 410-412

DOI:https://doi.org/10.1007/BF02635019· OSTI ID:4125555

Smith, F H

Details for the germination of cotton seedlings and their utilization for incorporating $sup 14$C-labeled acetate into gossypol are presented. Methods for the isolation and purification of the biosynthesized gossypol are given. The specific activity of the gossypol obtained from root tissue and cotyledons had mean values for 8 preparations of 428.6 x 10$sup 3$ and 6.3 x 10$sup 3$ dpm/mg, respectively. Corresponding incorporations were 5.42 and 0.32 percent. (auth)

• Research Organization: North Carolina State Univ., Raleigh
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-009645
• OSTI ID: 4125555
• Journal Information: J. Am. Oil Chem. Soc., v. 5, no. 9, pp. 410-412, Other Information: Orig. Receipt Date: 30-JUN-76
• Country of Publication: United States
• Language: English

Related Subjects

N48730* -Life Sciences-Nuclide Kinetics & Toxicology- Plants
550200* -Biomedical Sciences
Basic Studies-Biochemistry
*ACETATES- BIOCHEMICAL REACTION KINETICS
*COTTON- RADIONUCLIDE KINETICS
*POLYPHENOLS- CHEMICAL PREPARATION
*SEEDLINGS- GERMINATION
BIOSYNTHESIS
CARBON 14
LABELLED COMPOUNDS
ROOTS