Enzymatic repair of uv-irradiated DNA in vitro
Conference
·
OSTI ID:4111238
- ed.
Excision repair of uv-damaged Bacillus subtilis transforming DNA has been carried out by a sequential enzyme system in vitro. Incision adjacent to the pyrimidine dimer in the DNA strand by correndonuclease II-initiated excision of the damage by the 5' $Yields$ 3'-directed exonuclease of the Micrococcus luteus DNA polymerase. Reinsertion of nucleotides into the gap in the strand by the DNA polymerase at 10$sup 0$C terminated in a single-strand break which was sealed by a polynucleotide ligase, thereby repairing the DNA strand. This restored biological activity to damaged DNA up to doses resulting in 60 percent inactivation of transforming activity. At higher doses, less repair was achieved, due to the development of double-strand breaks during the in vitro incision and excision steps. (auth)
- Research Organization:
- Brandeis Univ., Waltham, MA
- NSA Number:
- NSA-33-012272
- OSTI ID:
- 4111238
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
*BACILLUS SUBTILIS-- BIOLOGICAL RADIATION EFFECTS
*DNA-- BIOLOGICAL REPAIR
*NUCLEASES-- BIOLOGICAL EFFECTS
*POLYMERASES-- BIOLOGICAL EFFECTS
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
DIMERS
MICROCOCCUS
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
NUCLEOTIDES
PYRIMIDINES
STRAND BREAKS
ULTRAVIOLET RADIATION
*DNA-- BIOLOGICAL REPAIR
*NUCLEASES-- BIOLOGICAL EFFECTS
*POLYMERASES-- BIOLOGICAL EFFECTS
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
DIMERS
MICROCOCCUS
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
NUCLEOTIDES
PYRIMIDINES
STRAND BREAKS
ULTRAVIOLET RADIATION