Molecular basis for the mutagenic and lethal effects of ultraviolet irradiation. Progress report, December 1, 1977--November 30, 1978. [Micrococcus luteus, Escherichia coli]

Technical Report · Sat Jul 01 04:00:00 EDT 1978

DOI:https://doi.org/10.2172/6620492· OSTI ID:6620492

Grossman, L

Our earlier work on the chemical basis of mutagenesis led to certain chemical generalities necessary to explain how certain mutagens such as UV light and hydroxylamine functioned in information transfer systems (replicative, transcriptive and translational). When such modifications were applied to biologically active DNA in a controlled manner biological expression was non-stoichiometric because much of the damage was removed from the DNA by repair systems. Our efforts were then directed to these systems which led to: the isolation, purification and characterization of endonucleases responsible for the first and controlling step in DNA repair referred to as incision in both M. luteus and E. coli; the isolation, purification and characterization of exonucleases responsible for the removal or excision of damaged nucleotides in M. luteus and human placental trophoblasts; the repair of UV damaged biologically active transforming and transfecting DNAs by purified endonucleases, exonucleases, DNA polymerase I and polynucleotide ligase from M. luteus and E. coli; the characterization of the dual gene control for incision phenomenon in M. luteus and E. coli; and isolation, purification and characterization of repair enzymes from human placenta.

• Research Organization: Johns Hopkins Univ., Baltimore, MD (USA). School of Hygiene and Public Health
• OSTI ID: 6620492
• Report Number(s): COO-2814-3
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
DOSES
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
FETAL MEMBRANES
HYDROXYLAMINE
LETHAL DOSES
LETHAL RADIATION DOSE
MEMBRANES
MICROCOCCUS
MICROCOCCUS LUTEUS
MICROORGANISMS
NUCLEASES
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHOTRANSFERASES
PLACENTA
POLYMERASES
PURIFICATION
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
TRANSFERASES
ULTRAVIOLET RADIATION