Effect of Irradiation of the Primer DNA on the Incorporation of Precursors
The rate of enzymatic incorporation of H3-labeled thymidine into primer DNA was studied after the DNA had been irradiated with doses up to 360,000 roentgens of rays. Nonirradiated DNA-synthesizing enzyme obtained from regenerating rat liver was used together with DNA in the incubation system. An initial increase followed by a decrease was noted in one sample of DNA, whereas a second lesshighly polymerized sample showed only a decrease in the rate of incorporation. Isolated calf-thymus nuclei showed no changes in the rate of incorporation of H3-labeled thymidine or cytidylic acid after irradiation with very high doses of rays. The results of these in vitro studies do not provide support for the concept that damage to DNA preventing replication is a factor in the decrease in DNAsynthesis noted in irradiated cells.
- Research Organization:
- Univ. of Rochester School of Medicine and Dentistry, N.Y.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-15-008550
- OSTI ID:
- 4104127
- Journal Information:
- International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, Vol. 3, Issue 1; Other Information: Orig. Receipt Date: 31-DEC-61; ISSN 0020-7616
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
Incorporation of Tritium-labelled Thymidine and Tritium-labelled Deoxycytidylic Acid into Deoxyribonucleic Acid after Irradiation of the Deoxyribonucleic Acid Primer with γ-Rays
Enzymatic assay for deoxyribonucleoside triphosphates using synthetic oligonucleotides as template primers