Metabolism of 1-$sup 14$C linolenic acid in developing brain. II. Incorporation of radioactivity from 1-$sup 14$C linolenate into brain lipids

Journal Article · Wed Jan 01 04:00:00 EST 1975 · Lipids, v. 10, no. 4, pp. 242-247

OSTI ID:4102019

Dhopeshwarkar, G A; Subramanian, C

Metabolism of 1-$sup 14$C linolenic acid was studied in growing animals by injecting the tracer intraperitoneally into 12-13 day old suckling rats and following up the results by sacrificing groups of animals at 8 hr, 48 hr, 15 day, and 45 day intervals. In the first 15 days, there was a greater decrease in radioactivity of brain total lipids compared to the later period, although the earlier age period is characterized by lipid deposition rather than breakdown. Results showed that 8 hr after the tracer phosphatidyl choline had the highest specific radioactivity. When the total phosphatidyl ethanolamine fraction was resolved into diacyl and alk-l-enyl species, it was found that radioactivity was not distributed evenly between the two species. There was a progressive increase in radioactivity of the alkenyl and a decrease in the diacyl species. Forty- eight hr after the tracer, however, the radioactivity of phosphatidyl ethanolamine increased and at 45 days remained slightly higher than phosphatidyl choline. Radioactivity of cholesterol, a result of synthesis from acetate undoubtedly derived from the breakdown of tracer linolenate, was also high 48 hr after tracer and remained high until 45 days. (auth)

• Research Organization: Univ. of California, Los Angeles
• NSA Number: NSA-33-015116
• OSTI ID: 4102019
• Journal Information: Lipids, v. 10, no. 4, pp. 242-247, Journal Name: Lipids, v. 10, no. 4, pp. 242-247; ISSN LPDSA
• Country of Publication: United States
• Language: English

Related Subjects

*BRAIN-- BIOCHEMICAL REACTION KINETICS
*CARBOXYLIC ACIDS-- BIOCHEMICAL REACTION KINETICS
*CHOLESTEROL-- BIOCHEMICAL REACTION KINETICS
*LINOLENIC ACID-- METABOLISM
*LIPIDS-- BIOCHEMICAL REACTION KINETICS
550601* --Biomedical Sciences
Basic Studies--Medicine-- Unsealed Radionuclides in Diagnostics
BIOSYNTHESIS
CARBON 14
N48830* --Life Sciences--New Tracer Techniques--Other
RADIOACTIVITY
RATS
TRACER TECHNIQUES