COLLECTING C$sup 14$$O$$sub 2$ IN A WARBURG FLASK FOR SUBSEQUENT SCINTILLATION COUNTING
Journal Article
·
· J. Lipid Research
OSTI ID:4071893
An improved technique for the collection of C/sup 1//sup 4/O/sub 2/ in a Warburg flask and for the subsequent transfer of the radioactive material for liquid scintillation assay is described. A high-molecular-weight quaternary amine is used for absorbing the radioactive carbon dioxide. The absorbant is injected into the center well or side arm of the flask, then sulfuric acid is poured into the main compartment to release the carbon dioxide from the solution, bicarbonate buffer gassed with 95% O/sub 2/, 5% CO/sub 2/, C/sup 1//sup 4/- labeled substrate. After shaking for 3 hrs at 37 the material is transferred to a glass counting vial that contains a toluene mixture, and the radioactive content is measured by a liquid scintillation spectrometer. (N.W.R.)
- Research Organization:
- Oak Ridge Inst. of Nuclear Studies, Tenn.
- NSA Number:
- NSA-15-020679
- OSTI ID:
- 4071893
- Journal Information:
- J. Lipid Research, Journal Name: J. Lipid Research Vol. Vol: 2: No. 2
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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