Mitigation of graft-versus-host disease in lethally irradiated mice grafted with spleen cells adherent to glass beads

Journal Article · Mon Mar 01 04:00:00 EST 1976 · Transplantation, v. 21, no. 3, pp. 247-254

DOI:https://doi.org/10.1097/00007890-197603000-00009· OSTI ID:4062230

Gambrill, M R; Ledney, G D; MacVittie, T J

Murine spleen cells were separated on the basis of adherence to glass beads into distinct subpopulations that differ in their ability to produce acute graft-versus-host disease (GVHD). Nonadherent CBA spleen cells produce acute GVHD in 6 to 10 days in lethally irradiated (C57BL/6 x CBA)F$sub 1$ mice as do unfractionated spleen cells. Spleen cells which are adherent to glass beads, however, enable 71 percent of the mice to survive without symptomatology of acute GVHD. The low proliferative response of these cells to phytohemagglutinin (PHA) correlated with the mitigated GVHD seen in animals grafted with this fraction. Proliferative cells as determined by the spleen colony assay and the in vitro agar colony-forming assay are present in this fraction as are cells responsive to mitogenic stimulation with lipopolysaccharide (LPS). B6CBF$sub 1$ mice grafted with CBA adherent cells exhibit a gradual return over a period of 5 months to normal PHA and LPS stimulation levels as shown by splenic cell responses of these mice to mitogens. Surviving mice grafted with adherent cells were chimeric as determined by electrophoretic hemoglobin pattern analysis and serial bone marrow transplantation. (auth)

• Research Organization: Armed Forces Radiobiology Research Inst., Bethesda, MD
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-029703
• OSTI ID: 4062230
• Journal Information: Transplantation, v. 21, no. 3, pp. 247-254, Journal Name: Transplantation, v. 21, no. 3, pp. 247-254; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

*GRAFT-HOST REACTION
*IMMUNE REACTIONS-- BIOLOGICAL RADIATION EFFECTS
*IMMUNOSUPPRESSION-- RADIOINDUCTION
560152* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Vertebrates
COBALT 60
GAMMA RADIATION
LETHAL IRRADIATION
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
SPLEEN CELLS