The Use of Chromium-51 as a Sensitive Quantitative Criterion of Early Radiation Damage to Rat Thymocytes

Journal Article · Tue Mar 31 23:00:00 EST 1964 · Canadian Journal of Biochemistry

DOI:https://doi.org/10.1139/o64-059· OSTI ID:4058489

Scaife, J. F.; Vittorio, P. V.

The uptake of ⁵¹Cr-chromate by thymocytes in suspension can be used as a sensitive quantitntive criterion for the evaluation of early radiation damage to these cells, often under situations where other methods of assessing cell death such as morphological changes do not yield valid results. The development of a radiation lesion is characterized by a reduction in the uptake of ⁵¹Cr, which in vitro can be detected one hr following irradiation and with doses as low as 25 rads. By means of this technique thymocytes have been shown to be completely protected by anoxia to doses of up to 150 rads, but at higher levels anoxia affords little protection. No protection of thymocytes could be demonstrated in vivo with S, BETA -aminoethylisothiuronium bromide hydrobromide (AET) or serotonin at 0.72 mmoles/kg L-cysteine, cysteamine, and serotonin at 1 mM concentration in vitro showed no protection of thymocytes provided they were removed from the cells immediately subsequent to irradiation.

• Research Organization: Defence Research Chemical Labs., Ottawa
• Sponsoring Organization: USDOE
• NSA Number: NSA-18-017504
• OSTI ID: 4058489
• Journal Information: Canadian Journal of Biochemistry, Journal Name: Canadian Journal of Biochemistry Journal Issue: 4 Vol. 42; ISSN 0008-4018
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AET
AMINES
AMINO ACIDS
ANIMAL CELLS
BIOLOGY AND MEDICINE
CHROMIUM 51
CHROMIUM OXIDES
CYSTEINE
DETECTION
EFFICIENCY
ETHYL RADICALS
IRRADIATION
LABELLED COMPOUNDS
MEA
METABOLISM
MONITORING
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDATION
PERFORMANCE
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RADIATION PROTECTION
RADIOSENSITIVITY
RATS
SENSITIVITY
SEROTONIN
SUSPENSIONS
THYMOCYTES
THYMUS
TISSUES
TRACER TECHNIQUES
USES
VARIATIONS