EFFECT OF CHLORAMPHENICOL ON THE BIOSYNTHESIS OF DNA IN X-IRRADIATED ESCHERICHIA COLI B

Journal Article · Sat Apr 01 04:00:00 EST 1961 · Biochem. Biophys. Research Communs.

DOI:https://doi.org/10.1016/0006-291X(61)90216-9· OSTI ID:4054331

Mitetic, B; Kucan, Z; Drakulic, M; Zajec, Lj

Escherichia coli grown in a mineral medium were exposed to various doses of x radiation. Chloramphenicol was added to half the irradiated suspension at a concentration of 10 mu g/ml. Desoxyribonucleic acid (DNA) levels were determined after chemical fractionation of the cells. Growth was observed to stop in both control and irradiated cultures after the addition of chloramphenicol. The synthesis of ribonucleic acid (RNA) continued, but was significantly inhibited in samples exposed to 16,000 r. Doses of 1000 r had no measurable effect on DNA synthesis and the addition of chloramphenicol did not effect the degradation of DNA or the inhibition of its biosynthesis. It is concluded that both the degradation of DNA and the effect of chloramphenicol on DNA metabolism after x irradiation depend on the degree to which the mechanism of DNA biosynthesis is damaged. (C.H.)

• Research Organization: Inst. "Ruder Bodkovic," Zagreb
• Sponsoring Organization: USDOE
• NSA Number: NSA-15-015424
• OSTI ID: 4054331
• Journal Information: Biochem. Biophys. Research Communs., Journal Name: Biochem. Biophys. Research Communs. Vol. Vol: 4
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANTIBIOTICS
BACTERIA
BIOLOGY AND MEDICINE
CHEMICAL REACTIONS
CHLORAMPHENICOL
METABOLISM
NUCLEIC ACIDS
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RIBONUCLEIC ACID
X RADIATION