Radiolysis of cytosine in dilute neutral aqueous solution

Journal Article · Thu Jan 15 04:00:00 EST 1976 · J. Phys. Chem., v. 80, no. 2, pp. 112-119

DOI:https://doi.org/10.1021/j100543a005· OSTI ID:4050131

Gorin, G; Ohno, N; Raff, L M

Cytosine, 0.1 to 10 mM, has been radiolyzed at constant dose rates in 0.02 M phosphate buffer, pH 7. If the pH is then adjusted to 3, the absorption of the radiolysis products at 275 nm becomes negligible, and the radiochemical decomposition yield G(--cyt) can be calculated from the decrease in absorbance. In oxygen-saturated solutions it is described by the expression (D* = dose in krad): G(--cyt) = 3.3 -- 0.024D* (to at least 40 percent decomposition). The radiolysis products react with the radicals derived from the solvent about one fifth as fast as cytosine does. The radiolysis products have appreciable absorbance at less than 260 nm and pH 3, and at 266 nm and pH 7 or ll. The spectral characteristics of the predominant products resemble those of known dihydrocytosine derivatives. In solutions containing greater than 1 mM cytosine and initially saturated with air, the oxygen is soon depleted; in absence of oxygen, different radiolysis products having a higher absorbance are formed. (auth)

• Research Organization: Oklahoma State Univ., Stillwater
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-025493
• OSTI ID: 4050131
• Journal Information: J. Phys. Chem., v. 80, no. 2, pp. 112-119, Journal Name: J. Phys. Chem., v. 80, no. 2, pp. 112-119; ISSN JPCHA
• Country of Publication: United States
• Language: English

Related Subjects

*CYTOSINE-- RADIOLYSIS
400600* --Chemistry--Radiation Chemistry
BIOLOGICAL RADIATION EFFECTS
CHEMICAL REACTION YIELD
DNA
GAMMA RADIATION
N40300* --Chemistry--Radiation Chemistry
N48100 --Life Sciences--Radiation Effects on Biochemicals
OXYGEN
PH VALUE
SPECTROPHOTOMETRY