Yields of radiation-induced base products in DNA: effects of DNA conformation and gassing conditions
Gas chromatography-mass spectrometry with selected-ion monitoring was used to measure the yields of radiation-induced base products in aqueous solutions of native or heat-denatured DNA irradiated in the dose range 20-100 Gy. These DNA solutions were saturated with nitrous oxide, nitrogen, air or 20% oxygen in nitrous oxide during irradiation. The products measured were as follows: 5,6-dihydrothymine; 5-hydroxy-5,6-dihydrothymine (thymine glycol); 5-hydroxy-5,6-dihydrocytosine; 5,6-dihydroxy-5,6-dihydrocytosine (cytosine glycol); 4,6-diamino-5-formamidopyrimidine; 7,8-dihydro-8-oxoadenine (8-hydroxyadenine); 2,6-diamino-4-hydroxy-5-formamidopyrimidine; and 7,8-dihydro-8-oxoguanine (8-hydroxyguanine). In oxygenated solutions, 5,6-dihydrothymine, 5-hydroxy-5,6-dihydrothymine and 5-hydroxy-5,6-dihydrocytosine were not formed. The yields of all products, other than 5,6-dihydrothymine, were greater in irradiated DNA samples from N2O-saturated solutions than from N2-saturated solutions.
- Research Organization:
- Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
- OSTI ID:
- 5681708
- Report Number(s):
- AD-A-231138/9/XAB; AFFRI-SR-90-27
- Resource Relation:
- Other Information: Pub. in International Jnl. of Radiation Biology , v58 n3 p397-415 1990
- Country of Publication:
- United States
- Language:
- English
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GLYCOLS
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MASS SPECTROSCOPY
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MIXTURES
NITROGEN COMPOUNDS
NITROGEN OXIDES
NONMETALS
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560120* - Radiation Effects on Biochemicals
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