Preservation of Mouse Bone Marrow at -79⁰ C. with Dimethyl Sulphoxide
Dimethyl sulfoxide was shown to protect mouse bone marrow from the effects of freezing to --79 deg C. After thawing, the protected cells retained 50 to 60% of their ability to synthesize lipids and proteins. It was also found that mouse bone marrow stored for one month at --79 deg C in the presence of dimethyl sulfoxide will prevent the death of lethally irradiated mice and is only slightly inferior to fresh marrow. The hematological response of lethally irradiated mice to fresh and preserved marrow was also studied. The leukocyte response of mice that received frozen marrow preserved with dimethyl sulfoxide was slightly better than the response obtained with 1 x 10/sup 6/ fresh cells. Blood leukocyte levels were normal, but the differential counts were abnormal. (P.C.H.)
- Research Organization:
- National Inst. for Medical Research, London
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-15-021981
- OSTI ID:
- 4049670
- Journal Information:
- Nature (London), Journal Name: Nature (London) Journal Issue: 4782 Vol. 190; ISSN 0028-0836
- Publisher:
- Nature Publishing Group
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
TRANSPLANTATION OF BONE MARROW AFTER PRESERVATION AT-70 C
RADIATION PROTECTION OF MICE WITH BONE MARROW AND SPLEEN PRESERVED AT LOW TEMPERATURE USING POLYVINYLPYRROLIDONE
PRESERVATION OF RADIATION RECOVERY FACTOR IN FROZEN MARROW
Journal Article
·
Mon Jul 01 00:00:00 EDT 1957
· Journal of Applied Physiology (U.S.) Changed to J. Appl. Physiol.: Respir., Environ. Exercise Physiol.
·
OSTI ID:4261118
RADIATION PROTECTION OF MICE WITH BONE MARROW AND SPLEEN PRESERVED AT LOW TEMPERATURE USING POLYVINYLPYRROLIDONE
Journal Article
·
Sat Feb 29 23:00:00 EST 1964
· Blood (U.S.)
·
OSTI ID:4036504
PRESERVATION OF RADIATION RECOVERY FACTOR IN FROZEN MARROW
Journal Article
·
Sat Nov 30 23:00:00 EST 1957
· Blood (U.S.)
·
OSTI ID:4325248