SPECTROPHOTOMETRIC EVALUATION OF RADIATION DAMAGE TO HEMOGLOBIN

Journal Article · Wed Jan 01 04:00:00 EST 1964 · Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.)

OSTI ID:4049393

Pravdina, K I

Solutions of rat and pigeon blood hemoglobin, obtained by hemolysis and centrifugation of washed erythrocytes, were x-irradiated for 1 to 120 min at rates of 700 r/min. A hypochromic effect was shown with irradiation increasing from 0 to 80 kr, with the value of epsilon (ratio oi irradiated to unirradiated) for rat oxyhemoglobin decreasing from 100 to 75 at 555 m mu and to 55 at 578 m mu . Pigeon hemoglobin was more radiosensitive than rat. The marked dependence of the change on the dose indicated that the absorption at 578 m mu might be used as a criterion of radiation damage. Irradiation of oxyhemoglobin sharply increased the absorption at 630 m mu , indicating an increase in the methemoglobin. However, values were too variable for this to be used as a criterion. With 56 kr irradiation, considerable change occurred in the protein part of the molecule, with appearance of globin-hemochromogen spectral lines. Oxyhemoglobin irradiated at 2 to 7 kr and incubated at room temperature in 0.019N NaOH was more susceptible to formation of these lines than the unirradiated. Oxygen effects and after-effects were observed in the irradiated solutions. (TTT)

• Research Organization: Inst. of Cytology, Leningrad
• NSA Number: NSA-18-017567
• OSTI ID: 4049393
• Journal Information: Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.), Journal Name: Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.) Vol. Vol: 4; ISSN RADOA
• Country of Publication: Country unknown/Code not available
• Language: Russian

Related Subjects

BIOLOGY AND MEDICINE
BIRDS
BLOOD
CENTRIFUGATION
DECOMPOSITION
ERYTHROCYTES
HEMOGLOBIN
HEMOLYSIS
IMMUNITY
IRRADIATION
OXIDATION
OXYGEN
PHOTOMETRY
PIGEONS
PROTEINS
RADIATION DOSES
RADIATION EFFECTS
RADIOSENSITIVITY
RATS
REACTION KINETICS
REDUCTION
SOLUTIONS
SPECTRA
SPECTROSCOPY
STANDARDS
VARIATIONS
X RADIATION