INDUCED MUTATION RATES WITH GAMMA RAYS AT A SPECIFIC LOCUS IN OATS
Journal Article
·
· Crop Science (U.S.)
Seeds of the Victorgain variety of oats, which are susceptible to the fungus Helminthosporium victoriae. were irradiated with Co/sup 60/ gamma rays and grown to maturity in isolation. The germinating seeds in the X/sub 2/ generation were treated with toxin produced by the fungus H. victoriae, and resistant seedlings were selected. These resistant seedlings represent induced mutations at the locus controlling susceptibility in the oat variety. When the seeds at 10% moisture were irradiated at 0. 9, l8, 27, and 36 kr at an intensity of 4.5 kr per minute, the induced mutation rates were 0, 10.4, 13.6, 0.9, and 5.1 x 10/sup -8/ per roentgen per locus, respectively. When the seed moisture was 33%, ob4.5, 9, and 13.6 kr at an intensity of 4.5 kr per minute, the locus, respectively. When the seeds with mositure of 10% were given goses of 17 kr and 25 kr at low intensities the induced mutation rates were 8.8 and 4.6 x10/sup -8// r/locus respectively. These induced mutation rates are in the same order of magnitude as those reported for mice and Drosophila, did not increase linearly with increasing dosage. were influenced by moisture content of the seeds at the time of irradiation. and were not influenced by the range in intensity tested (20 r/minute to 4.5 kr/minute). (auth)
- Research Organization:
- Florida Agricultural Experiment Station; and Crops Research Div., ARS, USDA, Gainesville, Fla.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-15-016826
- OSTI ID:
- 4039039
- Journal Information:
- Crop Science (U.S.), Journal Name: Crop Science (U.S.) Vol. Vol: 1; ISSN CRPSA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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