RECOVERY OF DEOXYRIBONUCLEIC ACID SYNTHESIS IN ULTRAVIOLET-LIGHT-EXPOSED BACTERIA

Journal Article · Wed Aug 23 04:00:00 EDT 1961 · Biochem. Biophys. Research Communs.

DOI:https://doi.org/10.1016/0006-291X(61)90050-X· OSTI ID:4011198

Doudney, C A

Studies of the effects of chloramphenicol on induced mutation and on nucleic acid synthesis in synchronized cultures of Esoherichia coli exposed to ultraviolet light demonstrated that a relation exists between the amount of postirradiation RNA which has been synthesized at the time of chloramphenicol addition and the relative rate of DNA synthesis in the presence of chloramphenicol. These studies were extended to log phase cultures of E. coli strain B/r, and experimental results are described which suggest that the recovery of cellular capacity for DNA synthesis (involving the synthesis of RNA and protein) is related to the institution of a rate limiting component of the postirradiation DNA synthetic systsm. The results further show that the presence of chloramphenicol after ultraviolet exposure does not limit the amount of DNA formed as is the case with unirradiated cells. (auth)

• Research Organization: Univ. of Texas, Houston
• Sponsoring Organization: USDOE
• NSA Number: NSA-15-025861
• OSTI ID: 4011198
• Journal Information: Biochem. Biophys. Research Communs., Journal Name: Biochem. Biophys. Research Communs. Vol. Vol: 5
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANTIBIOTICS
BACTERIA
BIOLOGY AND MEDICINE
BIOSYNTHESIS
CHLORAMPHENICOL
ESCHERICHIA COLI
MUTATIONS
NUCLEIC ACIDS
PROTEINS
QUANTITY RATIO
RECOVERY
RIBONUCLEIC ACID
ULTRAVIOLET RADIATION