Development of an in vitro rainbow trout cell bioassay for PCBs and dioxins
- Michigan State Univ., East Lansing, MI (United States)
- Univ. of California, Davis, CA (United States)
The toxicity of PCBs, dioxins, and other halogenated aromatic hydrocarbons (HAHS) is difficult to predict from chemical analysis alone, since these chemicals occur in complex mixtures and participate in interactions. Since HAH toxicity is correlated with affinity for the aromatic hydrocarbon receptor (AhR), bioassays which measure induction of genes controlled by the AhR can predict toxicity. The authors have developed a bioassay relevant to fish derived from the RTH-149 rainbow trout hepatoma cell line. The parent cell line has been stably transfected with the plasmid pGudLucl.l. This plasmid contains the firefly luciferase reporter gene under the control of mouse DRES. The reporter gene is controlled only by the AhR, and its induction can be detected at low levels. The bioassay will be fully characterized and calibrated to the responses of whole fish exposed to HAHS. The bioassay exhibits detectable induction at 100 fM TCDD after a three day exposure. Induction increases in a linear relationship with time up to four days of exposure. Luciferase activity was induced to 1 7.5 times background activity after four days of exposure to 1 nM TCDD.
- OSTI ID:
- 40037
- Report Number(s):
- CONF-9410273--
- Country of Publication:
- United States
- Language:
- English
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