An in vitro rainbow trout cell bioassay for AhR-mediated toxins

Conference · Sun Dec 31 04:00:00 EST 1995

OSTI ID:242317

Richter, C A; Giesy, J P ^[1]; Denison, M S ^[2]

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1. Michigan State Univ., E. Lansing, MI (United States)
2. Univ. of California, Davis, CA (United States)

The toxicity of PCBs, dioxins, and other halogenated aromatic hydrocarbons (HAHS) at environmentally relevant concentrations is in large part mediated through the aromatic hydrocarbon receptor (AhR). Bioassays which measure the activity of genes regulated by the receptor provide an integrative measure of the total AhR-mediated toxicity of a sample. The authors have recently developed and characterized a bioassay using recombinant rainbow trout hepatoma cells containing the firefly luciferase reporter gene under the regulation of the AhR. The cell line is designated Remodulated Lightning Trout (RLT). The RLT bioassay is relevant to fish, and is useful as a rapid screening device, a guide for chemical analysis, and a tool for studies of the AhR mechanism. The responses of the RLT cell line to various PCB congeners are similar to responses of in vivo fish bioassays. The authors now report on the responses of the bioassay to dioxins, dibenzofurans, and other related compounds as compared to in vivo fish bioassays. The authors will also report on the utility of the RLT bioassay in measuring the total TEQ of complex mixtures.

• OSTI ID: 242317
• Report Number(s): CONF-9511137--; ISBN 1-880611-03-1
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL INDICATORS
BIOLOGICAL MARKERS
DIOXIN
GENE REGULATION
HYDROCARBONS
LIVER CELLS
POLYCHLORINATED BIPHENYLS
RECEPTORS
SENSITIVITY
TOXICITY
TROUT
WATER POLLUTION