The effects of smoltification on the distribution, metabolism and elimination of benzo[a]pyrene in juvenile coho salmon, Oncorhynchus kisutch
Conference
·
OSTI ID:398229
- Simon Fraser Univ., Burnaby, British Columbia (Canada). Dept. of Biological Sciences
The activities of Phase 1 and Phase 2 biotransformation enzymes in the livers of yearling coho salmon (Oncorhynchus kisutch) were determined bi-weekly beginning in February until release from the hatchery in mid June, in order to observe alterations in baseline levels during smoltification. Peak activities occurred in February and March and then declined, Cytochrome P450 levels ranged from 5.27 {+-} 0.34 to 0.83 {+-} 0.29 nmol/g liver, EROD activities ranged from 352.19 {+-} 24.35 to 81.04 {+-} 11.63 pmol/min/g liver, and glutathione S-transferase activities ranged from 11.07 {+-} 0.377 to 3.77 {+-} 0.64 mmol/min/g liver. The whole body excretion {sup 3}H-benzo[a]pyrene (B[a]P) was examined following i.p. injection during this same period. A trend towards increased B[a]P retention occurred from February to April and then declined until June. The bile contained the largest amount of b[A]P-derived radioactivity per gram tissue weight, followed by the liver, kidney and fat. Bile analysis revealed that 55--63% of the radioactivity were Phase 1 metabolites. The remaining radioactivity detected was 16--24% glucuronide conjugates, 8% sulfate conjugates, 7% other organic conjugates and 6% aqueous-soluble metabolites. These findings suggest that the transformation from freshwater adapted coho parr to smolts significantly alters biotransformation enzyme activities and the distribution and elimination of xenobiotics such as benzo[a]pyrene.
- OSTI ID:
- 398229
- Report Number(s):
- CONF-9511137--; ISBN 1-880611-03-1
- Country of Publication:
- United States
- Language:
- English
Similar Records
The uptake, distribution and metabolism of benzo[a]pyrene in coho salmon (Oncorhynchus kisutch) during the parr-smolt transformation
The effects of smoltification on the uptake, distribution and metabolism of benzo[a]pyrene in coho salmon
Hormonal regulation of lipid metabolism in developing coho salmon, Oncorhynchus kisutch
Journal Article
·
Tue Jul 01 00:00:00 EDT 1997
· Environmental Toxicology and Chemistry
·
OSTI ID:514568
The effects of smoltification on the uptake, distribution and metabolism of benzo[a]pyrene in coho salmon
Conference
·
Sat Dec 30 23:00:00 EST 1995
·
OSTI ID:398228
Hormonal regulation of lipid metabolism in developing coho salmon, Oncorhynchus kisutch
Thesis/Dissertation
·
Mon Dec 31 23:00:00 EST 1984
·
OSTI ID:6792085