Screening the dopamine D{sub 1} receptor gene in 131 schizophrenics and eight alcoholics: Identification of polymorphisms but lack of functionally significant sequence changes
- Mayo Clinic/Foundation, Rochester, MN (United States); and others
To determine whether mutations in the D{sub 1} dopamine receptor (D{sub 1}DR) gene are associated with schizophrenia, the coding sequence was examined in 106 Caucasian, 11 African-American, 8 Asian, and 6 Native American patients. Approximately 350 kb of genomic sequence was screened by dideoxy fingerprinting, a method related to single strand conformational polymorphism (SSCP) analysis that detects virtually 100% of sequence changes. One polymorphism was identified in Asians and one in Caucasians, but neither altered 421 the amino acid sequence (Leu{sup 66}, and Ser{sup 421}, respectively). In addition, a previously reported polymorphism in the 5{prime} untranslated region of exon 2 at bp -48 was found to be common, with an allele frequency of approximately 40% in Caucasians of Western European descent. Based on the fact that no sequence changes of likely functional significance were identified, these data suggest that mutations affecting the structure of the D{sub 1} dopamine receptor protein are uncommon and are unlikely to contribute significantly to the genetic predisposition to schizophrenia. The D{sub 1}DR gene also was examined in eight alcoholics, including 3 African-Americans and 1 Native American, but no sequence changes were identified. 35 refs., 1 fig., 3 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 393900
- Journal Information:
- American Journal of Medical Genetics, Journal Name: American Journal of Medical Genetics Journal Issue: 2 Vol. 60; ISSN 0148-7299; ISSN AJMGDA
- Country of Publication:
- United States
- Language:
- English
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