Validation of an in vitro normal rainbow trout hepatocyte bioassay for halogenated aromatic hydrocarbons
Conference
·
OSTI ID:392318
- Harvest Research Associates, Calgary, Alberta (Canada)
- HydroQual Labs. Ltd., Calgary, Alberta (Canada)
The validity of in vitro cell culture models for the measurement of halogenated aromatic hydrocarbons (HAH) toxicity or the prediction of potential toxicity depends upon the ability of the mode reflect in vivo toxicity. HAH toxicity is directly related to the affinity for the aromatic hydrocarbon receptor (AhR) which controls cytochrome P45 activity. In the bioassay characterized, normal rainbow trout hepatocyte monolayer cultures (RTL-WI) are employed to measure the modulation of cytochrome P450 monooxygenase activity, using the ethoxyresorufin-O-deethylase (EROD) assay, following the exposure to betanaphthaflavone (BNF) and environmental samples for varying times. Data from this single plate hepatocyte study are compared with liver microsomal EROD activity from rainbow trout exposed to the same samples in vivo.
- OSTI ID:
- 392318
- Report Number(s):
- CONF-9511137--; ISBN 1-880611-03-1
- Country of Publication:
- United States
- Language:
- English
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