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Effect of feeding rate on biochemical measures and fate of a single oral dose of PCB in Arctic char

Conference ·
OSTI ID:392311
 [1]; ; ; ;  [2]
  1. Univ. of Manitoba, Winnipeg, Manitoba (Canada). Dept. of Zoology
  2. Dept. of Fisheries and Oceans, Winnipeg, Manitoba (Canada)
This study was done to determine the effects of reducing lipid stores and the resulting mobilization of associated contaminants. Individually tagged Arctic char (Saivelinus alpinus) were treated with single gavage doses of 6.1 ng/g {sup 14}C-3,3{prime}4,4{prime},5-pentachlorobiphenyl (PCB 126) in gelatin or vehicle (controls). Following treatment, the PCB was allowed 3 weeks to reach equilibrium distribution within the fish. After this 3 week period, treated and control groups of fish were fed one of three rations, (1.5%, 0.75% or 0.25% body wt/d). Fish were sampled at 0, 8, 24 and 48 weeks following the initiation of the different rations. PCB concentrations and lipid levels were measured in 5 tissues (muscle, liver, intestine, spleen and kidney). Biochemical response measured included hepatic EROD, retinoids and tocopherol. Growth was reduced in both treated and control groups fed the lowest ration. EROD activity was negatively correlated with growth and positively correlated with liver concentrations of PCB 126. Declines in mean lipid content were ration dependent. Changes in hepatic retinoids were related to both ration and treatment. The results indicate that a low ration resulted in greater utilization of stored lipids and higher tissue concentrations of contaminants compared to the other rations. These changes altered biochemical measures which are responsive to planar chlorinated aromatic hydrocarbons.
OSTI ID:
392311
Report Number(s):
CONF-9511137--; ISBN 1-880611-03-1
Country of Publication:
United States
Language:
English