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Title: Induction of sister chromatid exchange in the presence of gadolinium-DTPA and its reduction by dimethyl sulfoxide

Abstract

The authors investigate the frequency of sister chromatid exchange (SCE) after the addition of gadolinium (Gd)-DTPA to venous blood samples. Venous blood was obtained from nonsmokers. Samples were incubated with Gd-DTPA alone or in combination with mitomycin C, cytarabine, and dimethyl sulfoxide (DMSO), and then evaluated for SCEs. The frequency of SCE increased with the concentration of Gd-DTPA and as each chemotherapeutic agent was added. Sister chromatid exchange frequencies were lower when the blood was treated with a combination of Gd-DTPA and DMSO compared with Gd-DTPA alone. The increase in frequency of SCE seen after the addition of Gd-DTPA was decreased by the addition of DMSO, indicating the production of hydroxyl radicals. The effect likely is dissociation-related. 14 refs., 6 tabs.

Authors:
; ;  [1];  [2]
  1. Tokyo Medical and Dental Univ., Yokohama (Japan)
  2. Yokohama City Univ. (Japan)
Publication Date:
OSTI Identifier:
391300
Resource Type:
Journal Article
Resource Relation:
Journal Name: Investigative Radiology; Journal Volume: 31; Journal Issue: 5; Other Information: PBD: May 1996
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; HUMAN CHROMOSOMES; SISTER CHROMATID EXCHANGES; MUTATION FREQUENCY; GADOLINIUM; GENETIC EFFECTS; DMSO; DTPA; DNA; GENETIC RADIATION EFFECTS; DNA REPAIR; MUTATIONS; CHEMOTHERAPY; HYDROXYL RADICALS; RADIOPROTECTIVE SUBSTANCES; LYMPHOCYTES

Citation Formats

Yamazaki, Etsuo, Fukuda, Hozumi, Shibuya, Hitoshi, and Matsubara, Sho. Induction of sister chromatid exchange in the presence of gadolinium-DTPA and its reduction by dimethyl sulfoxide. United States: N. p., 1996. Web. doi:10.1097/00004424-199605000-00007.
Yamazaki, Etsuo, Fukuda, Hozumi, Shibuya, Hitoshi, & Matsubara, Sho. Induction of sister chromatid exchange in the presence of gadolinium-DTPA and its reduction by dimethyl sulfoxide. United States. doi:10.1097/00004424-199605000-00007.
Yamazaki, Etsuo, Fukuda, Hozumi, Shibuya, Hitoshi, and Matsubara, Sho. 1996. "Induction of sister chromatid exchange in the presence of gadolinium-DTPA and its reduction by dimethyl sulfoxide". United States. doi:10.1097/00004424-199605000-00007.
@article{osti_391300,
title = {Induction of sister chromatid exchange in the presence of gadolinium-DTPA and its reduction by dimethyl sulfoxide},
author = {Yamazaki, Etsuo and Fukuda, Hozumi and Shibuya, Hitoshi and Matsubara, Sho},
abstractNote = {The authors investigate the frequency of sister chromatid exchange (SCE) after the addition of gadolinium (Gd)-DTPA to venous blood samples. Venous blood was obtained from nonsmokers. Samples were incubated with Gd-DTPA alone or in combination with mitomycin C, cytarabine, and dimethyl sulfoxide (DMSO), and then evaluated for SCEs. The frequency of SCE increased with the concentration of Gd-DTPA and as each chemotherapeutic agent was added. Sister chromatid exchange frequencies were lower when the blood was treated with a combination of Gd-DTPA and DMSO compared with Gd-DTPA alone. The increase in frequency of SCE seen after the addition of Gd-DTPA was decreased by the addition of DMSO, indicating the production of hydroxyl radicals. The effect likely is dissociation-related. 14 refs., 6 tabs.},
doi = {10.1097/00004424-199605000-00007},
journal = {Investigative Radiology},
number = 5,
volume = 31,
place = {United States},
year = 1996,
month = 5
}
  • 3-Aminobenzamide (3AB) is a potent inhibitor of poly(ADP-ribose) synthesis and has been reported to inhibit DNA repair. Inhibition of poly(ADP-ribose) synthesis by 3AB results in a potentiation of the cytotoxic effects of alkylating agents and synergistically increases the frequency of sister chromatid exchanges (SCEs) induced in Chinese hamster ovary cells by methyl methanesulfonate (MMS). Experiments were carried out to determine if this synergism was related to an inhibition of the repair or the removal of SCE-forming lesions by 3AB. Cells were treated with MMS or ethyl nitrosourea (ENU) and either held in a nonproliferative state or maintained in exponential growthmore » for up to 48 hr before SCE frequencies were measured. During this time, the SCE frequency decreased greatly. 3AB did not affect this decrease in either cycling or noncycling cells. Furthermore, 3AB appears to exert its effect only in cycling cells when bromodeoxyuridine is present. Therefore, the synergism between 3AB and alkylating agents in the induction of SCEs is unrelated to effects of 3AB on the repair or removal of SCE-forming lesions.« less
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