Localization of the human estrogen-responsive finger protein (EFP) gene (ZNF147) within a YAC contig containing the myeloperoxidase (MPO) gene
The estrogen-responsive finger protein (EFP) gene (ZNF-147) belongs to a family of putative transcription factors that contain RING finger, B-box, and coiled-coil domains. Among this family are three genes, PML, RET, and T18, that play defined roles in carcinogenesis. EFP expression is induced by estrogen via an estrogen-responsive element (ERE) in the 3{prime}-untranslated region of the gene. The association between estradiol levels and breast/ovarian cancer risk makes an estrogen-responsive transcription factor such as EFP an attractive candidate for mediating this correlation. The chromosomal localization of EFP is also consistent with such a role. EFP previously was localized by fluorescence in situ hybridization (FISH) to human 17q21.3-q22 and mouse 11C, consistent with known linkage conservation between these regions. Recently, the human gene localization was refined to 17q23.1. This coincides with a region of chromosomal deletion identified by loss of heterozygosity (LOH) analysis in breast and ovarian tumors. To facilitate analysis of EFP involvement in breast and ovarian tumorigenesis, we isolated yeast artificial chromosome (YAC) and bacteriophage P1 clones encompassing the human and mouse EFP loci and developed a series of PCR primer pairs that amplify DNA encoding EFP functional domains. 15 refs., 1 fig.
- OSTI ID:
- 390949
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 28; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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