Definition of the tumor protein D52 (TPD52) gene family through cloning of D52 homologues in human (hD53) and mouse (mD52)

Journal Article · Thu Aug 01 04:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0393· OSTI ID:465988

Byrne, J A; Basset, P ^[1]; Mattei, M G ^[2]

---

1. Institut de Genetique et de Biologie Moleculaire et Cellulaire, Strasbourg (France)
2. Faculte de la Medecine de la Timone, Marseille (France)

Cloning is reported of a cDNA homologue to the breast carcinoma-associated D52 cDNA, termed D53, and of a mouse D52 cDNA (HGMW-approved symbols TPD52L1 and TPD52). Human D53 and mouse D52 proteins are predicted to be 52 and 86% identical to human D52, respectively. Analysis of the three protein sequences identified a coiled-coil domain and N- and C-terminally located PEST domains in each. The conservation of homology between the D52 and the D53 sequences, combined with a lack of homology between these and known proteins, defines a new mammalian gene/protein family, the D52 family. The human D52 locus has been previously mapped to chromosome 8q21, and using in situ mapping in the present study, a human D53 locus was mapped to chromosome 6q22-q23. We observed coexpression of the human D52 and D53 genes in some breast tumors and derivative cell lines and found that maintenance of D52 and D53 transcript levels in estrogen receptor-positive MCF7 breast carcinoma cells depends upon estradiol. However, D52 and D53 genes were specifically expressed in HL-60 and K-562 leukemia cells, respectively, with 12-O-tetradecanoylphorbol-13-acetate treatment decreasing D52 and D53 transcript levels in these cell lines. The presence of a coiled-coil domain, combined with observed codependent or independent expression of the D52 and D53 genes, suggests that D52 and D53 proteins may be capable of hetero- and/or homodimer formation. 41 refs., 5 figs., 1 tab.

• OSTI ID: 465988
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 35; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
BANDING TECHNIQUES
CARCINOMAS
CELL DIFFERENTIATION
DIMERS
DNA
DNA SEQUENCING
DNA-CLONING
ESTRADIOL
GENE REGULATION
GENES
GENETIC EFFECTS
GENETIC MAPPING
HUMAN CHROMOSOME 6
HUMAN CHROMOSOME 8
IN-SITU HYBRIDIZATION
LEUKEMIA
MAMMARY GLANDS
MICE
NUCLEOTIDES
PROTEINS
TRANSCRIPTION
TUMOR CELLS