Definition of the tumor protein D52 (TPD52) gene family through cloning of D52 homologues in human (hD53) and mouse (mD52)
- Institut de Genetique et de Biologie Moleculaire et Cellulaire, Strasbourg (France)
- Faculte de la Medecine de la Timone, Marseille (France)
Cloning is reported of a cDNA homologue to the breast carcinoma-associated D52 cDNA, termed D53, and of a mouse D52 cDNA (HGMW-approved symbols TPD52L1 and TPD52). Human D53 and mouse D52 proteins are predicted to be 52 and 86% identical to human D52, respectively. Analysis of the three protein sequences identified a coiled-coil domain and N- and C-terminally located PEST domains in each. The conservation of homology between the D52 and the D53 sequences, combined with a lack of homology between these and known proteins, defines a new mammalian gene/protein family, the D52 family. The human D52 locus has been previously mapped to chromosome 8q21, and using in situ mapping in the present study, a human D53 locus was mapped to chromosome 6q22-q23. We observed coexpression of the human D52 and D53 genes in some breast tumors and derivative cell lines and found that maintenance of D52 and D53 transcript levels in estrogen receptor-positive MCF7 breast carcinoma cells depends upon estradiol. However, D52 and D53 genes were specifically expressed in HL-60 and K-562 leukemia cells, respectively, with 12-O-tetradecanoylphorbol-13-acetate treatment decreasing D52 and D53 transcript levels in these cell lines. The presence of a coiled-coil domain, combined with observed codependent or independent expression of the D52 and D53 genes, suggests that D52 and D53 proteins may be capable of hetero- and/or homodimer formation. 41 refs., 5 figs., 1 tab.
- OSTI ID:
- 465988
- Journal Information:
- Genomics, Vol. 35, Issue 3; Other Information: PBD: 1 Aug 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
GENES
DNA-CLONING
DNA SEQUENCING
GENETIC MAPPING
GENE REGULATION
TRANSCRIPTION
MAMMARY GLANDS
CARCINOMAS
HUMAN CHROMOSOME 8
HUMAN CHROMOSOME 6
ESTRADIOL
GENETIC EFFECTS
TUMOR CELLS
CELL DIFFERENTIATION
PROTEINS
MICE
NUCLEOTIDES
DNA
IN-SITU HYBRIDIZATION
BANDING TECHNIQUES
DIMERS
LEUKEMIA
AMINO ACID SEQUENCE