High-resolution comparative mapping of the proximal region of the mouse X chromosome
- MRC Radiobiology Unit, Oxon (United Kingdom)
- The Wellcome Trust Centre for Human Genetics, Oxford (United Kingdom); and others
The murine homologues of the loci for McLeod syndrome (XK), Dent`s disease (ClCN5), and synaptophysin (SYP) have been mapped to the proximal region of the mouse X chromosome and positioned with respect to other conserved loci in this region using a total of 948 progeny from two separate Mus musculus x Mus spretus backcrosses. In the mouse, the order of loci and evolutionary breakpoints (EB) has been established as centromere-(DXWas70, DXHXF34h)-EB-Clen5-(Syp, DXMit55, DXMit26)-Tfe3-Gata1-EB-Xk-Cybb-telomere. In the proximal region of the human X chromosome short arm, the position of evolutionary breakpoints with respect to key loci has been established as DMD-EB-XK-PFC-EB-GATA1-C1CN5-EB-DXS1272E-ALAS2-EB-DXF34-centromere. These data have enabled us to construct a high-resolution genetic map for the {approximately}3-cM interval between DXWas70 and Cybb on the mouse X chromosome, which encompasses 10 loci. This detailed map demonstrates the power of high-resolution genetic mapping in the mouse as a means of determining locus order in a small chromosomal region and of providing an accurate framework for the construction of physical maps. 31 refs., 4 figs., 1 tab.
- OSTI ID:
- 390936
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 28; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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