Molecular cloning of the human homolog of a striatum-enriched phosphatase (STEP) gene and chromosomal mapping of the human and murine loci
- Stanford Univ. Medical Center, CA (United States)
A gene for a protein tyrosine phosphatase (PTPase) was isolated from a human fetal brain cDNA library by PCR amplification. Sequence analysis revealed that the PTPase has a single phosphatase catalytic domain located at the C-terminus that includes the highly conserved amino acid domain [I/V]HCXAGXXR[S/T]GX[F/Y] found in all tyrosine phosphatases. Two proline-rich regions located at the N-terminus may contain putative Src homology domain 3 (SH3) binding motifs. Comparison of the PTPase with a previously cloned striatum enriched phosphatase (STEP) from rat and from mouse exhibited a high degree of identity ({approximately}85-90%) at both the nucleotide and the amino acid levels, indicating that the human PTPase is the homolog of the rat and murine STEP gene. By using a combination of somatic cell hybrid analysis and fluorescence in situ hybridization, we have mapped the human STEP locus to chromosome 11p15.2-p15.1 and the murine STEP gene to chromosome 7B3-B5. These are two regions of known conserved synteny, providing further evidence that the human STEP is a true homolog of the murine STEP gene. Candidate disease genes in the vicinity include Usher syndrome type 1C in human and a mouse mutant locus, twister (twt). 50 refs., 3 figs.
- OSTI ID:
- 390666
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 28; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
Similar Records
Structure of the murine MPTP-PEST gene: Genomic organization and chromosomal mapping
Molecular cloning of cDNAs encoding human GLEPP1, a membrane protein typrosine phosphatase: Characterization of the GLEPP1 protein distribution in human kidney and assignment of the GLEPP1 gene to human chromosome 12p12-p13
Protein tyrosine phosphatase containing SH2 domains: Characterization, preferential expression in hematopoietic cells, and localization to human chromosome 12p12-p13
Journal Article
·
Thu Aug 10 00:00:00 EDT 1995
· Genomics
·
OSTI ID:390674
Molecular cloning of cDNAs encoding human GLEPP1, a membrane protein typrosine phosphatase: Characterization of the GLEPP1 protein distribution in human kidney and assignment of the GLEPP1 gene to human chromosome 12p12-p13
Journal Article
·
Mon May 01 00:00:00 EDT 1995
· Genomics
·
OSTI ID:114900
Protein tyrosine phosphatase containing SH2 domains: Characterization, preferential expression in hematopoietic cells, and localization to human chromosome 12p12-p13
Journal Article
·
Fri Jan 31 23:00:00 EST 1992
· Molecular and Cellular Biology; (United States)
·
OSTI ID:7200962