Induction and phosphorylation of protein kinase C-{alpha} and mitogen-activated protein kinase by hypoxia and by radiation in Chinese hamster V79 cells
- Medical Research Council, Oxon (United Kingdom)
- Imperial Cancer Research Fund, London (United Kingdom)
Protein kinase C (PKC) and mitogen-activated protein (MAP) kinase are protein-serine/threonine kinases which are important regulators of diverse cellular processes including metabolism, proliferation and differentiation. This study shows that both hypoxia and X irradiation of serum-deprived Chinese hamster V79 cells cause the induction and phosphorylation of the PKC-{alpha} isoform. The increased induction and phosphorylation of PKC occur mainly in the nuclear fraction. Unlike the PKC activator TPA, neither hypoxic nor radiation stress causes translocation of PKC-{alpha} from the cytosol to the membrane. The induction of PKC-{alpha} by hypoxia is accompanied by an increased expression of MAP kinase but, in contrast, this does not occur when PKC-{alpha} is induced by radiation. Radiation, like TPA, causes a complete redistribution of MAP kinase from the cytosol to the nucleus. 28 refs., 7 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 387310
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 2 Vol. 145; ISSN 0033-7587; ISSN RAREAE
- Country of Publication:
- United States
- Language:
- English
Similar Records
The regulation of GRP78 and messenger RNA levels by hypoxia is modulated by protein kinase C activators and inhibitors
Activation of distinct protein kinase C isozymes by phorbol esters: Correlation with induction of interleukin 1. beta. gene expression
Mutation of a protein kinase C phosphorylation site in the erbB protein of avian erythroblastosis virus
Journal Article
·
Thu Mar 31 23:00:00 EST 1994
· Radiation Research
·
OSTI ID:56768
Activation of distinct protein kinase C isozymes by phorbol esters: Correlation with induction of interleukin 1. beta. gene expression
Journal Article
·
Tue Apr 18 00:00:00 EDT 1989
· Biochemistry; (USA)
·
OSTI ID:5668138
Mutation of a protein kinase C phosphorylation site in the erbB protein of avian erythroblastosis virus
Journal Article
·
Sat Oct 01 00:00:00 EDT 1988
· J. Virol.; (United States)
·
OSTI ID:5983605