[Measurement of mutagenesis to study the effects of chemical agents]. Final report, August 1, 1993--July 31, 1994

Technical Report · Sat Dec 31 04:00:00 EST 1994

DOI:https://doi.org/10.2172/382449· OSTI ID:382449

Puck, T T

This is the final report of a study conducted at the Eleanor Roosevelt Institute for Cancer Research, Inc. This study looked at mutagenesis as a measurement of the effects of chemical agents. Topics discussed in this report include: development of a new theory for the role of lipids and lipoproteins in the interactions of macromolecules; the action of caffeine in synergizing mutagenesis of agents like ionizing radiation by inhibition of cellular repair processes which was incorporated into a rapid procedure for detection of mutagenicity with high sensitivity; quantitative theoretical analysis of the mutagenesis process in cells exposed to physical and chemical mutagenic agents; theoretical analysis was developed leading to the conclusion that the visible chromosomal lesions described will also include a significant proportion of point mutations; application of this methodology for meaningful measurement of mutagenesis to study the effects of chemical agents was begun; and investigation of the cell cytoskeleton`s effect of genome exposure operating in the course of the differentiation process.

• Research Organization: Los Alamos National Lab., NM (United States); Eleanor Roosevelt Inst., Denver, CO (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 382449
• Report Number(s): LA-SUB--96-110; ON: DE96014731
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL EFFECTS
CAFFEINE
CELL DIFFERENTIATION
CELL MEMBRANES
CHROMOSOME BREAKAGE
CYTOLOGY
HAZARDOUS MATERIALS
IONIZING RADIATIONS
LIPIDS
LIPOPROTEINS
MEASURING METHODS
MICROTUBULES
MUTAGENESIS
PROGRESS REPORT