Molecular mechanism of null expression of aldehyde dehydrogenase-1 in rat liver

Journal Article · Mon Apr 01 04:00:00 EST 1996 · Biochemical Genetics

DOI:https://doi.org/10.1007/BF02396244· OSTI ID:381294

Chen, J; Yoshida, Akira ^[1]; Yanagawa, Yuchio ^[2]

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1. Institute of the City of Hope, Duarte, CA (United States)
2. Tokohu Univ., Sendai (Japan)

In isozyme systems in general, the pattern of tissue-dependent expression of a given type of isozyme is uniform in various mammalian species. In contrast, a major cytosolic aldehyde dehydrogenase isozyme, termed ALDH1, which is strongly expressed in the livers of humans and other mammals, is hardly detectable in rat liver. Thirteen nucleotides existing in the 5{prime}-promoter region of human, marmoset, and mouse ALDH1 genes are absent in the four rat strains examined. When the 13 nucleotides were deleted from a chloramphenicol acetyltransferase expression construct, which contained the 5{prime} promoter region of the human ALDH1 gene and a low-background promoterless chloramphenicol acetyltransferase expression vector, the expression activity was severely diminished in human hepatic cells. Thus, deletion of the 13 nucleotides in the promoter region of the gene can account for the lack of ALDH1 expression in rat liver. 16 refs., 3 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 381294
• Journal Information: Biochemical Genetics, Journal Name: Biochemical Genetics Journal Issue: 3-4 Vol. 34; ISSN 0006-2928; ISSN BIGEBA
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ALDEHYDES
DNA SEQUENCING
GENE REGULATION
GENES
NUCLEOTIDES
OXIDOREDUCTASES
PROTEINS
STRUCTURE-ACTIVITY RELATIONSHIPS
TISSUE DISTRIBUTION
TRANSCRIPTION