Developmental toxicity of copper chloride, methylene chloride, and 6-aminonicotinamide to embryos of the grass shrimp Palaemonetes pugio
Journal Article
·
· Environmental Toxicology and Chemistry
- Jacksonville State Univ., AL (United States). Dept. of Biology
- Environmental Protection Agency, Gulf Breeze, FL (United States). National Health and Environmental Effects Research Lab.
Embryos of estuarine grass shrimp Palaemonetes pugio have demonstrated sensitivity to various solvents and petroleum products, indicating utility for evaluating estuarine contamination. Testing was performed to establish concentration-response curves for methylene chloride, copper chloride, and 6-aminonicotinamide, three known teratogenic chemicals. Two exposure periods were used, 4 d and 12 d, and both periods extended through hatching. The average 4-d LC50 values for methylene chloride, copper chloride, and 6-aminonicotinamide were 0.071% v/v, 1.82 mg/L, and 0.21 mg/ml, respectively. The average 12-d LC50 values for methylene chloride, copper chloride, and 6-aminonicotinamide were 0.031% v/v, 1.44 mg/L, and 0.057 mg/ml, respectively. Eye malformations were observed with embryos exposed to concentrations greater than 3 mg/L copper chloride or greater than 0.07% v/v methylene chloride. Very few abnormalities were observed in embryos exposed to 6-aminonicotinamide. Abnormal larval development was found with exposure to copper chloride at concentrations greater than 1 mg/L. The sensitivity and low variability found here further supports the development of these relatively simple methods using grass shrimp embryos. Establishment of sublethal developmental endpoints warrants further investigation because of their potential correspondence to mechanisms of toxic action.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 361941
- Journal Information:
- Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 5 Vol. 18; ISSN 0730-7268; ISSN ETOCDK
- Country of Publication:
- United States
- Language:
- English
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