Analysis of tumor suppressor gene on human chromosome 9 in mouse x human somatic cell hybrids

Journal Article · Thu Sep 01 04:00:00 EDT 1994 · Somatic Cell and Molecular Genetics

DOI:https://doi.org/10.1007/BF02257456· OSTI ID:35719

Porterfield, B W; Olopade, O I; Rowley, J D; Diaz, M O

Deletions of the short arm of human chromosome 9 (9p) are common in human leukemia and solid tumors. The minimum region of overlap of these deletions, located between the interferon genes and the methylthioadenosine phosphorylase gene, is partially synthenic with a region of mouse chromosome 4 that has tumor suppressor activity. Somatic cell hybrids between tumorigenic, MTAP-deficient, mouse L cells, and MTAP-competent human cells containing either a normal copy of 9p or a 9p with a deletion involving band 9p21 were selected in culture conditions that require MTAP activity for continued growth. Somatic cell hybrids that contained a normal copy of 9p rarely formed tumors in nude mice. Cells from the rare tumors that grew had lost the normal 9p. Hybrid cells that contained a 9p with deletions formed tumors more frequently, and cells from these tumors retained the 9p deletion chromosome. These results provide evidence that a tumor suppressor gene (or genes) is located on human chromosome 9 within the region of deletion.

• Sponsoring Organization: USDOE
• OSTI ID: 35719
• Journal Information: Somatic Cell and Molecular Genetics, Journal Name: Somatic Cell and Molecular Genetics Journal Issue: 5 Vol. 20; ISSN 0740-7750; ISSN SCMGDN
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CHROMOSOMAL ABERRATIONS
GENETIC CONTROL
HUMAN CHROMOSOME 9
LEUKEMIA
MICE
NEOPLASMS