DNA sequencing with capillary electrophoresis and single cell analysis with mass spectrometry

Technical Report · Fri Mar 27 04:00:00 EST 1998

DOI:https://doi.org/10.2172/348902· OSTI ID:348902

Fung, N

Since the first demonstration of the laser in the 1960`s, lasers have found numerous applications in analytical chemistry. In this work, two different applications are described, namely, DNA sequencing with capillary gel electrophoresis and single cell analysis with mass spectrometry. Two projects are described in which high-speed DNA separations with capillary gel electrophoresis were demonstrated. In the third project, flow cytometry and mass spectrometry were coupled via a laser vaporization/ionization interface and individual mammalian cells were analyzed. First, DNA Sanger fragments were separated by capillary gel electrophoresis. A separation speed of 20 basepairs per minute was demonstrated with a mixed poly(ethylene oxide) (PEO) sieving solution. In addition, a new capillary wall treatment protocol was developed in which bare (or uncoated) capillaries can be used in DNA sequencing. Second, a temperature programming scheme was used to separate DNA Sanger fragments. Third, flow cytometry and mass spectrometry were coupled with a laser vaporization/ionization interface.

• Research Organization: Ames Lab., IA (United States)
• Sponsoring Organization: USDOE Office of Energy Research, Washington, DC (United States)
• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 348902
• Report Number(s): IS-T--1841; ON: DE99002506
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CELL FLOW SYSTEMS
DNA SEQUENCERS
DNA SEQUENCING
ELECTROPHORESIS
EXPERIMENTAL DATA
MASS SPECTROSCOPY
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE