Method for the production of dicarboxylic acids
The present invention is an economical fermentation method for the production of carboxylic acids comprising the steps of (a) inoculating a medium having a carbon source with a carboxylic acid-producing organism; (b) incubating the carboxylic acid-producing organism in an aerobic atmosphere to promote rapid growth of the organism thereby increasing the biomass of the organism; (c) controllably releasing oxygen to maintain the aerobic atmosphere; (d) controllably feeding the organism having increased biomass with a solution containing the carbon source to maintain the concentration of the carbon source within the medium of about 0.5 g/l up to about 1 g/l; (e) depriving the aerobic atmosphere of oxygen to produce an anaerobic atmosphere to cause the organism to undergo anaerobic metabolism; (f) controllably feeding the organism having increased biomass a solution containing the carbon source to maintain the concentration of the carbon source within the medium of {>=}1 g/l; and (g) converting the carbon source to carboxylic acids using the anaerobic metabolism of the organism. 7 figs.
- Research Organization:
- Lockheed Martin Energy Research Corporation; Argonne National Laboratory (ANL), Argonne, IL
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-31109-ENG-38; AC05-96OR22464
- Assignee:
- Lockhead Martin Energy Research Corp., Oak Ridge, TN (United States)
- Patent Number(s):
- US 5,869,301/A/
- Application Number:
- PAN: 8-792,655
- OSTI ID:
- 321241
- Country of Publication:
- United States
- Language:
- English
Mutants of Escherichia coli deficient in the fermentative lactate dehydrogenase.
|
journal | January 1989 |
Carbon metabolism regulates expression of the pfl (pyruvate formate-lyase) gene in Escherichia coli.
|
journal | January 1991 |
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