Construction of a bioluminescent reporter strain to detect polychlorinated biphenyls
Journal Article
·
· Applied and Environmental Microbiology
OSTI ID:302310
- Univ. of Tennessee, Knoxville, TN (United States)
A bioluminescent reporter strain, Ralstonia eutropha ENV307 (pUTK60), was constructed for the detection of polychlorinated biphenyls by inserting the biphenyl promoter upstream of the bioluminescence genes. In the presence of a nonionic surfactant, which enhances the solubility of chlorinated biphenyls, bioluminescence was induced three- to fourfold over background by biphenyl, monochlorinated biphenyls, and Aroclor 1242. The minimum detection limits for these compounds ranged from 0.15 mg/liter for 4-chlorobiphenyl to 1.5 mg/liter for Aroclor 1242.
- Sponsoring Organization:
- USDOE, Washington, DC (United States); Tennessee Univ., Knoxville, TN (United States)
- OSTI ID:
- 302310
- Journal Information:
- Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 12 Vol. 64; ISSN AEMIDF; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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