Data for "Metabolic Engineering Strategies to Produce Medium-Chain Oleochemicals via Acyl-ACP:CoA Transacylase Activity"

Dataset · Thu Feb 24 00:00:00 EST 2022

DOI:https://doi.org/10.13012/B2IDB-4936526_V1· OSTI ID:3015970

Yan, Qiang ^[1]; Cordell, William ^[1]; Jindra, Michael ^[1]; Pfleger, Brian ^[2]

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1. Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Wisconsin-Madison, Madison, WI, 53706, USA
2. Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Wisconsin-Madison, Madison, WI, 53706, USA; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI, 53706, USA

Microbial lipid metabolism is an attractive route for producing oleochemicals. The predominant strategy centers on heterologous thioesterases to synthesize desired chain-length fatty acids. To convert acids to oleochemicals (e.g., fatty alcohols, ketones), the narrowed fatty acid pool needs to be reactivated as coenzyme A thioesters at cost of one ATP per reactivation – an expense that could be saved if the acyl-chain was directly transferred from ACP- to CoA-thioester. Here, we demonstrate such an alternative acyl-transferase strategy by heterologous expression of PhaG, an enzyme first identified in Pseudomonads, that transfers 3-hydroxy acyl-chains between acyl-carrier protein and coenzyme A thioester forms for creating polyhydroxyalkanoate monomers. We use it to create a pool of acyl-CoA’s that can be redirected to oleochemical products. Through bioprospecting, mutagenesis, and metabolic engineering, we develop three strains of Escherichia coli capable of producing over 1 g/L of medium-chain free fatty acids, fatty alcohols, and methyl ketones.

• Research Organization: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of Illinois Urbana-Champaign
• Sponsoring Organization: U.S. Department of Energy (DOE)
• DOE Contract Number: SC0018420
• OSTI ID: 3015970
• Country of Publication: United States
• Language: English

Related Subjects

Bioproducts
Metabolomics