Natural variation in the consequences of gene overexpression during osmotic stress [BAR-Seq]
- GLBRC - University of Wisconsin-Madison
We conducted a barcode sequencing experiment examining 4 yeast strains expressing a high copy number plasmid library. For each strain, we took samples at generation 0 (start of experiment) and generation 10 to quantify how plasmid abundance changes over time when strains were grown under osmotic and ionic stress induced by 0.7M of sodium chloride (NaCl). Each strain was measured in biological triplicate for a total of 24 samples.
- Research Organization:
- Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- DOE Contract Number:
- SC0018409
- OSTI ID:
- 3003537
- Country of Publication:
- United States
- Language:
- English
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