Natural variation in the consequences of gene overexpression and its implications for evolutionary trajectories

Robinson, DeElegant; Place, Michael; Hose, James; Jochem, Adam; Gasch, Audrey P.

doi:10.7554/eLife.70564

Natural variation in the consequences of gene overexpression and its implications for evolutionary trajectories

Journal Article · Mon Aug 02 00:00:00 EDT 2021 · eLife

DOI:https://doi.org/10.7554/eLife.70564· OSTI ID:1811587

^[1]; Place, Michael ^[2]; Hose, James ^[3]; Jochem, Adam ^[3]; ^[4]

Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, United States
Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, United States
Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, United States
Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, United States, Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, United States, Department of Medical Genetics, University of Wisconsin-Madison, Madison, United States

Copy number variation through gene or chromosome amplification provides a route for rapid phenotypic variation and supports the long-term evolution of gene functions. Although the evolutionary importance of copy-number variation is known, little is understood about how genetic background influences its tolerance. Here, we measured fitness costs of over 4000 overexpressed genes in 15 Saccharomyces cerevisiae strains representing different lineages, to explore natural variation in tolerating gene overexpression (OE). Strain-specific effects dominated the fitness costs of gene OE. We report global differences in the consequences of gene OE, independent of the amplified gene, as well as gene-specific effects that were dependent on the genetic background. Natural variation in the response to gene OE could be explained by several models, including strain-specific physiological differences, resource limitations, and regulatory sensitivities. This work provides new insight on how genetic background influences tolerance to gene amplification and the evolutionary trajectories accessible to different backgrounds.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: SC0018409

OSTI ID:: 1811587

Alternate ID(s):: OSTI ID: 1811588
OSTI ID: 1852646

Journal Information:: eLife, Journal Name: eLife Vol. 10; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
copy number variation
gene overexpression
genetics and genomics
natural variation
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Natural variation in the consequences of gene overexpression and its implications for evolutionary trajectories

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