Physical and genetic maps for chromosome 10

Journal Article · Sat May 01 04:00:00 EDT 1993 · Genomics

DOI:https://doi.org/10.1006/geno.1993.1192· OSTI ID:28961

Lichter, J B; Difilippantonio, M J; Pakstis, A J; Ward, D C; Kidd, K K ^[1]; Goodfellow, P J ^[2]

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1. Yale Univ., New Haven, CT (United States)
2. Univ. of British Columbia, Vancouver (Canada)

A fluorescence in situ hybridization (FISH) physical map of 14 polymorphic loci on chromosome 10 covers over 62% of the fractional length of chromosome 10. The positions of three previously mapped loci are confirmed, nine more are refined, and two new loci are cytogenetically mapped. The order of loci determined by FISH agrees with that obtained by genetic linkage studies. When the distance estimates for the physical map are compared to the distance estimates of the existing linkage map, the sex average ratio of the fractional length (FL) per centimorgan (cM) for this portion of chromosome 10 is 0.004 (or 0.4% FL/cM). However, the average ratios for male- and female-specific genetic distances are quite different, in agreement with an overall higher rate of recombination in females (0.008 FL/cM and 0.003 FL/cM, respectively). Moreover, the ratio across the centromere is larger for both the male (0.031 FL/cM) and the female (0.009 FL/cM) than the ratio encompassing the q arm (0.006 FL/cM for males and 0.002 FL/cM for females), suggesting that there is reduced recombination at the centromere in both the male and the female maps when compared to the physical distance generated from FISH on metaphase chromosomes. 15 refs., 3 figs., 1 tab.

• OSTI ID: 28961
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 16; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
DNA HYBRIDIZATION
GENETIC MAPPING
HUMAN CHROMOSOMES
SEX DEPENDENCE