Coexisting amplifications of the chromosome 1p32 genes (PTPRF and MYCL1) encoding protein tyrosine phosphatase LAR and L-myc in a small cell lung cancer line
- Univ. of British Columbia, Vancouver (Canada)
The L-myc proto-oncogene has been localized to human chromosome 1p32. The gene (MYCL1) encoding this transcription factor is amplified in a subset of human small cell lung cancer (SCLC) tumors and cell lines. The LAR protein tyrosine phosphatase gene (PTPRF) was also localized to chromosome 1p32, adjacent to the 1p32-p33 boundary. LAR is a receptor-like molecule expressed primarily in epithelial tissues. The tandem fibronectin type-III motifs present within the extracellular region have suggested that LAR may have a role in regulating cell adhesion. To determine whether the PTPRF and MYCL1 genes might be involved in simultaneous amplification events, we examined several SCLC cell lines for the presence of PTPRF gene amplifications. Three lines containing MYCL1 gene amplifications were analyzed. EcoRI-digested DNA ({approximately}10 {mu}g loaded per lane) from these lines was probed with the {sup 32}P-labeled L-myc cDNA probe: a 1.8-kb SmaI to EcoRI genomic fragment derived from the human MYCL1 gene. The hybridization intensity of the fragments seen in the autoradiogram suggested varying degrees of MYCL1 gene amplification as previously reported for H510A, H889, and H1092. This EcoRI restriction fragment length polymorphism has been observed with high frequency in the general population. 14 refs., 2 figs.
- OSTI ID:
- 273450
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 27; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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