Direct physical measure of conformational rearrangement underlying potassium channel gating
In response to membrane depolarization, voltage-grated ion channels undergo a structural rearrangement that moves charges or diapoles in the membrane electric field and opens the channel-conducting pathway. By combination of site-specific fluorescent labeling of the Shaker potassium channel protein with voltage clamping, this gating conformational charge was measured in real time. Curing channel activation, a stretch of at least seven amino acids of the putative transmembrane segment S4 moved from a buried postions into the extracellular environment. This movement correlated with the displacement of the gating charge, providing physical evidence in support of the hypothesis that S4 is the voltage sensor of voltage-gated ion channels. 24 refs., 4 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 263056
- Journal Information:
- Science, Journal Name: Science Journal Issue: 5246 Vol. 271; ISSN SCIEAS; ISSN 0036-8075
- Country of Publication:
- United States
- Language:
- English
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