Different susceptibility to lung tumorigenesis in mice with an identical Kras 2 intron 2

Journal Article · Wed Sep 20 04:00:00 EDT 1995 · Genomics

DOI:https://doi.org/10.1006/geno.1995.9984· OSTI ID:258282

Manenti, G; Falvella, F S; Gariboldi, M; Dragani, T A; Pierotti, M A ^[1]

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1. Instituto Nazionale Tumori, Milan (Italy)

The A/J mouse strain is genetically susceptible to pulmonary tumorigenesis. We have performed a genetic linkage analysis to map pulmonary adenoma susceptibility (Pas) loci in an urethane-treated (A/J X Mus spretus) x C57BL/6J (ASB) interspecific testcross. In this interspecific cross we have confirmed our previous results in AC3F2 mice on the mapping of the Pas1 locus to the digital region of chromosome 6, near Kras2. The A/J and M. spretus strains differed as the Pas1 locus, with the M. spretus providing the resistant allele. In the latter strain, we studied the nucleotide sequence of a portion of the second intron of Kras2 that contains polymorphisms associated with lung tumor susceptibility in several inbred strains. The lung tumor-resistant M. spretus strain had the same specific nucleotide sequence of susceptible strains. Mutations in codon 61 of Kras2 in urethane-induced lung tumors from ASF1 hybrids involved the A/J allele in all cases, while the M. spretus allele was never affected. Our results indicate that the M. spretus and A/J mice have an identical structure of the second intron of the Kras2 gene, but they differ in genetic susceptibility to pulmonary tumorigenesis and in mutability of their Kras2 allele. 30 refs., 4 figs., 3 tabs.

• Sponsoring Organization: USDOE
• OSTI ID: 258282
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 29; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ADENOMAS
CHROMOSOMES
CODONS
DISEASE RESISTANCE
DNA SEQUENCING
GENE MUTATIONS
GENES
GENETIC MAPPING
GENETICS
HYBRIDIZATION
INTRONS
LUNGS
MICE
NUCLEOTIDES
STATISTICS