Cloning and sequencing of cDNA encoding the human ribosomal protein L11 mRNA
Journal Article
·
· Russian Journal of Bioorganic Chemistry
OSTI ID:255165
- Novosibirsk Inst. of Bioorganic chemistry (Russian Federation); and others
To clone the RPL11 cDNA, we used a polymerase chain reaction (PCR) with the single-stranded cDNA synthesized on the total placentary poly(A){sup +}mRNA with the use of primer M245 containing a 3{prime}-terminal oligo(dT)-tract, the 5{prime}terminal hexadecanucleotide sequence of the M13 universal primer, and a NotiI restriction site between them. On the basis of the known sequence of the 5{prime}-end of the human ribosomal protein L11 mRNA, we chose two partially overlapping deoxyribooligonucleotides as 5{prime}-terminal primers in the amplification of the RPL11 cDNA. A pair of partially overlapping oligonucleotides complementary to the oligo(dT)-containing primer were used as 3{prime}-terminal primers.
- OSTI ID:
- 255165
- Journal Information:
- Russian Journal of Bioorganic Chemistry, Journal Name: Russian Journal of Bioorganic Chemistry Journal Issue: 2 Vol. 21; ISSN RJBCET; ISSN 1068-1620
- Country of Publication:
- United States
- Language:
- English
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