Mapping the gene that encodes phosphatidylinositol-specific phospholipase C-{gamma}2 in the human and the mouse

Journal Article · Thu Sep 15 04:00:00 EDT 1994 · Genomics

DOI:https://doi.org/10.1006/geno.1994.1533· OSTI ID:255154

Hernandez, D; Yulug, I G; Fisher, E M.C. ^[1]; Egan, S E ^[2]

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1. St. Mary`s Hospital Medical School, London (United Kingdom)
2. The Hospital for Sick Children, Toronto, Ontario (Canada)

The authors have mapped the PLCG2 gene, which encodes the enzyme phosphatidyl inositol-specific phospholipase C-{gamma}2. This is one of the phospolipases responsible for catalyzing the hydrolysis of phosphatidyl inositol in response to a great many mitogenic stimuli. PL C-{gamma}2 is an essential component of the signal transduction pathway between tyrosine kinases and downstream events such as protein kinase C activation and intracellular calcium release. The authors assigned PLCG2 to human chromosome 16 by amplification within a somatic cell hybrid mapping panel. To position the locus at a much finer resolution, PLCG2 sequences were amplified from a chromosome 16-specific somatic cell hybrid panel, which placed the gene on the long arm of the chromosome in band 16q24.1, a region that has few known genes. The authors have hybridized a mouse Plcg2 open reading frame probe to mouse DNAs from the European interspecific Backcross. The segregation pattern reveals the mouse Plcg2 locus maps to distal chromosome 8. 16 refs., 3 figs., 1 tab.

• OSTI ID: 255154
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 23; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 16
LIPASES
PHOSPHOTRANSFERASES