Probe for selectively characterizing enzymes involved in xenobiotic metabolism and method of making and using the same

Wright, Aaron T.; Ramos-Hunter, Susan; Whidbey, Christopher

Probe for selectively characterizing enzymes involved in xenobiotic metabolism and method of making and using the same

Patent · Tue Feb 06 04:00:00 EST 2024

OSTI ID:2541789

Wright, Aaron T.; Ramos-Hunter, Susan; Whidbey, Christopher

Activity-based probes that can be used to selectively identify and characterize enzymes that are involved in different phases of xenobiotic metabolism in a host and its microbiota population(s) are described. The activity-based probes described specifically label only their target active enzymes involved in xenobiotic metabolism and therefore provide a measurement of true protein functional activity rather than transcript or protein abundance. The activity-based probes also provide multimodal profiling of these active enzymes. Methods for preparing the activity based probes and exemplary methods for their use also are disclosed.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

Assignee:: Batelle Memorial Institute (Richland, WA)

Patent Number(s):: 11,891,655

Application Number:: 17/730,004

OSTI ID:: 2541789

Country of Publication:: United States

Language:: English

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