Fluorescence-based resource for semiautomated genomic analyses using microsatellite markers
- Johns Hopkins Medical Institutions, Baltimore, MD (United States); and others
To facilitate the practical application of highly-efficient semiautomated methods for general application in genomic analyses, the authors have developed a fluorescence-based microsatellite marker resource. Ninety highly polymorphic microsatellite markers were combined to provide a rapid, accurate, and highly efficient initial genome-wide screening system. These markers are spaced on average every 33 cM, with a mean heterozygosity of 81% (range 65-94%), covering 22 autosomes and the X and Y chromosomes. Less than 10% of the genome lies beyond 20 cM of the nearest marker. Since this genomic analysis system is fully compatible with automated fragment analyzers using simultaneous four-color fluorescence-based detection systems, the 5 groups of 18 markers can be detected concurrently. This multiplex detection provides a throughput of 1944 genotypes daily per instrument. This system will be highly beneficial in a number of clinical and research applications including linkage, cancer genetics, forensics, and cytogenetics. 16 refs., 1 fig., 2 tabs.
- OSTI ID:
- 250266
- Journal Information:
- Genomics, Vol. 24, Issue 2; Other Information: PBD: 15 Nov 1994
- Country of Publication:
- United States
- Language:
- English
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