Refinement of the localization of the gene for neuronal nicotinic acetylcholine receptor {alpha}4 subunit (CHRNA4) to human chromosome 20q13.2-q13.3
- Univ. of Heidelberg (Germany); and others
Using a human fetal brain genomic library and a P1 clone library from Genome Systems Inc, a cosmid and P1-clone walk has been initiated from the polymorphic markers D20S19, D20S20, D20S24, and D20S73. Standard methods were follwed for culture and preparation of the cosmids and P1 clones. Restriction mapping was performed by hybridization of T3 and T7 oligonucleotides to partial EcoRI/total NotI digests. A total of 43 cosmids and 4 P1 clones covering 420 kb in three contigs were isolated.
- OSTI ID:
- 250008
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 22; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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