Predicting the Identities of su(met-2) and met-3 in Neurospora crassa by Genome Resequencing

Journal Article · Thu Feb 01 00:00:00 EST 2024 · Fungal Genetics Reports (Online)

DOI:https://doi.org/10.4148/1941-4765.2183· OSTI ID:2483830

McCluskey, Kevin ^[1]; Brown, Daren W. ^[2]; Bredeweg, Erin ^[3]; Baker, Scott E. ^[3]

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1. Kansas State Univ., Manhattan, KS (United States); San Jose State Univ. Research Foundation, CA (United States)
2. US Dept. of Agriculture (USDA), Peoria, IL (United States). Agricultural Research Service (ARS)
3. Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

A significant number of classical genetic Neurospora crassa biochemical mutants remain anonymous, unassociated with a physical genome locus. By utilizing short read next-generation sequencing methods, it is possible to sequence the genomes of mutant strains rapidly and economically for the purpose of identifying genes associated with mutant phenotypes. We have taken this approach to connect genes and mutations to “methionineless” phenotypes in N. crassa.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER)
• Grant/Contract Number: AC02-05CH11231; AC05-76RL01830
• OSTI ID: 2483830
• Report Number(s): PNNL-SA--194065
• Journal Information: Fungal Genetics Reports (Online), Journal Name: Fungal Genetics Reports (Online) Journal Issue: 1 Vol. 67; ISSN 1941-4765
• Publisher: New Prairie Press
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Neurospora
cysteine
genome resequencing
metabolism
methionine
mutants