Identifying the gluc-1 and gluc-2 mutations in Neurospora crassa by genome resequencing

McCluskey, Kevin; Baker, Scott E.

doi:10.1007/s12041-022-01394-7

Identifying the gluc-1 and gluc-2 mutations in Neurospora crassa by genome resequencing

Journal Article · Wed Oct 26 00:00:00 EDT 2022 · Journal of Genetics

DOI:https://doi.org/10.1007/s12041-022-01394-7· OSTI ID:1972349

McCluskey, Kevin ^[1]; ^[2]

Bolt Threads, Emeryville, CA (United States)
Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)

Genome resequencing is an efficient strategy for associating mutant phenotypes with physical genomic loci. A pilot study of this approach demonstrated that the Neurospora crassa genetic map was critical in narrowing the possible candidate mutations in a strain to a small number in a limited, defined region of the genome. Here in this study, we utilize a resequencing strategy to identify the mutations underlying the gluc-1 and gluc-2 genes in N. crassa.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; AC05-76RL01830

OSTI ID:: 1972349

Report Number(s):: PNNL-SA-176923

Journal Information:: Journal of Genetics, Journal Name: Journal of Genetics Journal Issue: 2 Vol. 101; ISSN 0022-1333

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
beta-glucosidase
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genotype
neurospora crassa
phenotype
resequencing

Identifying the gluc-1 and gluc-2 mutations in Neurospora crassa by genome resequencing

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