Metallic Cation-Mediated Entrapment of Nucleic Acids on Mesoporous Silica Surface: Application in Castration-Resistant Prostate Cancer

Maestas-Olguin, Angelea; Olewine, Marian; Thabata, Sheymah; Tsala Ebode, Johanna; Arcos, Mariella; Krawchuck, Jacob; Coker, Eric N.; Brearley, Adrian J.; Xue, Xiang; Watt, John; Noureddine, Achraf; Brinker, C. Jeffrey

doi:10.1021/acs.chemmater.3c01174

Metallic Cation-Mediated Entrapment of Nucleic Acids on Mesoporous Silica Surface: Application in Castration-Resistant Prostate Cancer

Journal Article · Mon Dec 11 00:00:00 EST 2023 · Chemistry of Materials

DOI:https://doi.org/10.1021/acs.chemmater.3c01174· OSTI ID:2472587

^[1]; Olewine, Marian ^[1]; Thabata, Sheymah ^[1]; Tsala Ebode, Johanna ^[1]; Arcos, Mariella ^[1]; Krawchuck, Jacob ^[2]; ^[3]; Brearley, Adrian J. ^[1]; Xue, Xiang ^[1]; ^[4]; ^[1]; ^[1]

Univ. of New Mexico, Albuquerque, NM (United States)
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Center for Integrated Nanotechnologies
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

The use of exogenous nucleic acid technologies to modulate aberrant protein expression resulting from genetic mutations is a promising therapeutic approach for the treatment of diseases such as advanced prostate cancer (PC). The promise of nucleic-based therapeutics is dependent on the development of platforms that effectively protect nucleic acids from nuclease degradation and deliver the nucleic acids to the cytosol of target cells. In this work, we present the development of a divalent metal-mediated nucleic acid entrapment strategy with a porous silica matrix. This simple strategy results in efficient loading percentages of both siRNA (>60%) and mRNA (>80%) as well as their release within relevant biological environments (80%). Additionally, our data supports that the current method reduces endosomal entrapment and supports the lipid coating of mesoporous silica nanoparticles (LC-MSNs). Finally, the metal-enhanced nanosystem is assessed for biocompatibility, stability, and circulation within in vitro, ex ovo, and in vivo models of PC.

View Accepted Manuscript (DOE)

Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: Center for Metals and Biology and Medicine; National Aeronautics and Space Administration (NASA); National Cancer Institute (NCI); National Institutes of Health (NIH); National Science Foundation (NSF); USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: 89233218CNA000001; NA0003525

OSTI ID:: 2472587

Report Number(s):: LA-UR--23-33127

Journal Information:: Chemistry of Materials, Journal Name: Chemistry of Materials Journal Issue: 24 Vol. 35; ISSN 0897-4756

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

References (41)

Sol–Gel‐Based Advanced Porous Silica Materials for Biomedical Applications Lei, Qi; Guo, Jimin; Noureddine, Achraf Advanced Functional Materials, Vol. 30, Issue 41 https://doi.org/10.1002/adfm.201909539	journal	April 2020
Mesoporous Silica and Organosilica Nanoparticles: Physical Chemistry, Biosafety, Delivery Strategies, and Biomedical Applications Croissant, Jonas G.; Fatieiev, Yevhen; Almalik, Abdulaziz Advanced Healthcare Materials, Vol. 7, Issue 4 https://doi.org/10.1002/adhm.201700831	journal	November 2017
Self‐Sealing Porous Silicon‐Calcium Silicate Core–Shell Nanoparticles for Targeted siRNA Delivery to the Injured Brain Kang, Jinyoung; Joo, Jinmyoung; Kwon, Ester J. Advanced Materials, Vol. 28, Issue 36 https://doi.org/10.1002/adma.201600634	journal	July 2016
Efficient Functional Delivery of siRNA using Mesoporous Silica Nanoparticles with Ultralarge Pores Na, Hee-Kyung; Kim, Mi-Hee; Park, Kihyun Small, Vol. 8, Issue 11 https://doi.org/10.1002/smll.201200028	journal	March 2012
Protocells: Modular Mesoporous Silica Nanoparticle-Supported Lipid Bilayers for Drug Delivery Butler, Kimberly S.; Durfee, Paul N.; Theron, Christophe Small, Vol. 12, Issue 16 https://doi.org/10.1002/smll.201502119	journal	January 2016
“Integrative sol–gel chemistry”: a nanofoundry for materials science Faustini, Marco; Grosso, David; Boissière, Cédric Journal of Sol-Gel Science and Technology, Vol. 70, Issue 2 https://doi.org/10.1007/s10971-014-3321-9	journal	March 2014
Engineering of large-pore lipid-coated mesoporous silica nanoparticles for dual cargo delivery to cancer cells Noureddine, Achraf; Hjelvik, Elizabeth A.; Croissant, Jonas G. Journal of Sol-Gel Science and Technology, Vol. 89, Issue 1 https://doi.org/10.1007/s10971-018-4772-1	journal	August 2018
Engineering of monosized lipid-coated mesoporous silica nanoparticles for CRISPR delivery Noureddine, Achraf; Maestas-Olguin, Angelea; Saada, Edwin A. Acta Biomaterialia, Vol. 114 https://doi.org/10.1016/j.actbio.2020.07.027	journal	September 2020
Liposomal drug delivery systems: From concept to clinical applications Allen, Theresa M.; Cullis, Pieter R. Advanced Drug Delivery Reviews, Vol. 65, Issue 1 https://doi.org/10.1016/j.addr.2012.09.037	journal	January 2013
Nonviral cancer gene therapy: Delivery cascade and vector nanoproperty integration Zhou, Zhuxian; Liu, Xiangrui; Zhu, Dingcheng Advanced Drug Delivery Reviews, Vol. 115 https://doi.org/10.1016/j.addr.2017.07.021	journal	June 2017
Lipid nanoparticles for nucleic acid delivery: Current perspectives Samaridou, Eleni; Heyes, James; Lutwyche, Peter Advanced Drug Delivery Reviews, Vol. 154-155 https://doi.org/10.1016/j.addr.2020.06.002	journal	January 2020
RNAi-based therapeutics and tumor targeted delivery in cancer Kara, Goknur; Calin, George A.; Ozpolat, Bulent Advanced Drug Delivery Reviews, Vol. 182 https://doi.org/10.1016/j.addr.2022.114113	journal	March 2022
A mesoporous silica nanoparticle – PEI – Fusogenic peptide system for siRNA delivery in cancer therapy Li, Xu; Chen, Yijie; Wang, Mingqi Biomaterials, Vol. 34, Issue 4 https://doi.org/10.1016/j.biomaterials.2012.10.072	journal	January 2013
Hepsin promotes prostate cancer progression and metastasis Klezovitch, Olga; Chevillet, John; Mirosevich, Janni Cancer Cell, Vol. 6, Issue 2 https://doi.org/10.1016/j.ccr.2004.07.008	journal	August 2004
Cell surface expression of the stress response chaperone GRP78 enables tumor targeting by circulating ligands Arap, Marco A.; Lahdenranta, Johanna; Mintz, Paul J. Cancer Cell, Vol. 6, Issue 3 https://doi.org/10.1016/j.ccr.2004.08.018	journal	September 2004
Integrative genomic and proteomic analysis of prostate cancer reveals signatures of metastatic progression Varambally, Sooryanarayana; Yu, Jianjun; Laxman, Bharathi Cancer Cell, Vol. 8, Issue 5 https://doi.org/10.1016/j.ccr.2005.10.001	journal	November 2005
Prostate Cancer Cells Alter the Nature of Their Calcium Influx to Promote Growth and Acquire Apoptotic Resistance Monteith, Gregory R. Cancer Cell, Vol. 26, Issue 1 https://doi.org/10.1016/j.ccr.2014.06.015	journal	July 2014
Structural Alterations Driving Castration-Resistant Prostate Cancer Revealed by Linked-Read Genome Sequencing Viswanathan, Srinivas R.; Ha, Gavin; Hoff, Andreas M. Cell, Vol. 174, Issue 2 https://doi.org/10.1016/j.cell.2018.05.036	journal	July 2018
A Somatically Acquired Enhancer of the Androgen Receptor Is a Noncoding Driver in Advanced Prostate Cancer Takeda, David Y.; Spisák, Sándor; Seo, Ji-Heui Cell, Vol. 174, Issue 2 https://doi.org/10.1016/j.cell.2018.05.037	journal	July 2018
Partners in crime: deregulation of AR activity and androgen synthesis in prostate cancer Knudsen, Karen E.; Penning, Trevor M. Trends in Endocrinology & Metabolism, Vol. 21, Issue 5 https://doi.org/10.1016/j.tem.2010.01.002	journal	May 2010
Silica-Based Nanoparticles for Biomedical Applications: From Nanocarriers to Biomodulators Yang, Yannan; Zhang, Min; Song, Hao Accounts of Chemical Research, Vol. 53, Issue 8 https://doi.org/10.1021/acs.accounts.0c00280	journal	July 2020
Cellular Uptake of Nanoparticles versus Small Molecules: A Matter of Size Mosquera, Jesús; García, Isabel; Liz-Marzán, Luis M. Accounts of Chemical Research, Vol. 51, Issue 9 https://doi.org/10.1021/acs.accounts.8b00292	journal	August 2018
Lipid Nanoparticle Technology for Clinical Translation of siRNA Therapeutics Kulkarni, Jayesh A.; Witzigmann, Dominik; Chen, Sam Accounts of Chemical Research, Vol. 52, Issue 9 https://doi.org/10.1021/acs.accounts.9b00368	journal	August 2019
Lipids and Lipid Derivatives for RNA Delivery Zhang, Yuebao; Sun, Changzhen; Wang, Chang Chemical Reviews, Vol. 121, Issue 20 https://doi.org/10.1021/acs.chemrev.1c00244	journal	July 2021
Photoresponsive Nanovehicle for Two Independent Wavelength Light-Triggered Sequential Release of P-gp shRNA and Doxorubicin To Optimize and Enhance Synergistic Therapy of Multidrug-Resistant Cancer Wu, Ming; Lin, Xinyi; Tan, Xionghong ACS Applied Materials & Interfaces, Vol. 10, Issue 23 https://doi.org/10.1021/acsami.8b03823	journal	May 2018
Overcoming Chemoresistance in Cancer via Combined MicroRNA Therapeutics with Anticancer Drugs Using Multifunctional Magnetic Core–Shell Nanoparticles Yin, Perry T.; Pongkulapa, Thanapat; Cho, Hyeon-Yeol ACS Applied Materials & Interfaces, Vol. 10, Issue 32 https://doi.org/10.1021/acsami.8b09086	journal	July 2018
Dual Delivery of HNF4α and Cisplatin by Mesoporous Silica Nanoparticles Inhibits Cancer Pluripotency and Tumorigenicity in Hepatoma-Derived CD133-Expressing Stem Cells Tsai, Ping-Hsing; Wang, Mong-Lien; Chang, Jen-Hsuan ACS Applied Materials & Interfaces, Vol. 11, Issue 22 https://doi.org/10.1021/acsami.9b04474	journal	May 2019
Lipid Nanoparticles─From Liposomes to mRNA Vaccine Delivery, a Landscape of Research Diversity and Advancement Tenchov, Rumiana; Bird, Robert; Curtze, Allison E. ACS Nano, Vol. 15, Issue 11 https://doi.org/10.1021/acsnano.1c04996	journal	June 2021
Bridging the In Vitro to In Vivo gap: Using the Chick Embryo Model to Accelerate Nanoparticle Validation and Qualification for In Vivo studies Butler, Kimberly S.; Brinker, C. Jeffrey; Leong, Hon Sing ACS Nano, Vol. 16, Issue 12 https://doi.org/10.1021/acsnano.2c03990	journal	December 2022
Future of Mesoporous Silica Nanoparticles in Nanomedicine: Protocol for Reproducible Synthesis, Characterization, Lipid Coating, and Loading of Therapeutics (Chemotherapeutic, Proteins, siRNA and mRNA) Noureddine, Achraf; Maestas-Olguin, Angelea; Tang, Lien ACS Nano, Vol. 17, Issue 17 https://doi.org/10.1021/acsnano.3c07621	journal	August 2023
Mesoporous Silica Nanoparticle-Supported Lipid Bilayers (Protocells) for Active Targeting and Delivery to Individual Leukemia Cells Durfee, Paul N.; Lin, Yu-Shen; Dunphy, Darren R. ACS Nano, Vol. 10, Issue 9 https://doi.org/10.1021/acsnano.6b02819	journal	July 2016
Polyethyleneimine Coating Enhances the Cellular Uptake of Mesoporous Silica Nanoparticles and Allows Safe Delivery of siRNA and DNA Constructs Xia, Tian; Kovochich, Michael; Liong, Monty ACS Nano, Vol. 3, Issue 10 https://doi.org/10.1021/nn900918w	journal	September 2009
Fingerprinting the circulating repertoire of antibodies from cancer patients Mintz, Paul J.; Kim, Jeri; Do, Kim-Anh Nature Biotechnology, Vol. 21, Issue 1 https://doi.org/10.1038/nbt774	journal	December 2002
The molecular biology of prostate cancer: current understanding and clinical implications Gandhi, Jason; Afridi, Adil; Vatsia, Sohrab Prostate Cancer and Prostatic Diseases, Vol. 21, Issue 1 https://doi.org/10.1038/s41391-017-0023-8	journal	December 2017
mRNA-based therapeutics: powerful and versatile tools to combat diseases Qin, Shugang; Tang, Xiaoshan; Chen, Yuting Signal Transduction and Targeted Therapy, Vol. 7, Issue 1 https://doi.org/10.1038/s41392-022-01007-w	journal	May 2022
The Onpattro story and the clinical translation of nanomedicines containing nucleic acid-based drugs Akinc, Akin; Maier, Martin A.; Manoharan, Muthiah Nature Nanotechnology, Vol. 14, Issue 12 https://doi.org/10.1038/s41565-019-0591-y	journal	December 2019
Lipid nanoparticles for mRNA delivery Hou, Xucheng; Zaks, Tal; Langer, Robert Nature Reviews Materials, Vol. 6, Issue 12 https://doi.org/10.1038/s41578-021-00358-0	journal	August 2021
Lipid-coated mesoporous silica nanoparticles for anti-viral applications via delivery of CRISPR-Cas9 ribonucleoproteins LaBauve, Annette E.; Saada, Edwin A.; Jones, Iris K. A. Scientific Reports, Vol. 13, Issue 1 https://doi.org/10.1038/s41598-023-33092-4	journal	April 2023
Mesoporous silica nanoparticles: importance of surface modifications and its role in drug delivery Natarajan, Siva Kumar; Selvaraj, Stalin RSC Advances, Vol. 4, Issue 28 https://doi.org/10.1039/c4ra00781f	journal	January 2014
Lysosomal Acidification Mechanisms Mindell, Joseph A. Annual Review of Physiology, Vol. 74, Issue 1 https://doi.org/10.1146/annurev-physiol-012110-142317	journal	March 2012
The Chicken Embryo Model: A Novel and Relevant Model for Immune-Based Studies Garcia, Paul; Wang, Yan; Viallet, Jean Frontiers in Immunology, Vol. 12 https://doi.org/10.3389/fimmu.2021.791081	journal	November 2021

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60 APPLIED LIFE SCIENCES
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Metallic Cation-Mediated Entrapment of Nucleic Acids on Mesoporous Silica Surface: Application in Castration-Resistant Prostate Cancer

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