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Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting

Journal Article · · Nature Communications
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  1. Univ. of Vienna (Austria); OSTI
  2. Univ. of Vienna (Austria)
  3. Helmholtz Centre for Environmental Research, Leipzig (Germany)
  4. Eidgenoessische Technische Hochschule (ETH), Zurich (Switzerland)
  5. Univ. of Vienna (Austria); Aalborg Univ. (Denmark)
  6. Univ. of Vienna (Austria); Technical Univ. of Munich (Germany)
+ Show Author Affiliations

Prebiotics are defined as non-digestible dietary components that promote the growth of beneficial gut microorganisms. In many cases, however, this capability is not systematically evaluated. Here, we develop a methodology for determining prebiotic-responsive bacteria using the popular dietary supplement inulin. We first identify microbes with a capacity to bind inulin using mesoporous silica nanoparticles functionalized with inulin. 16S rRNA gene amplicon sequencing of sorted cells revealed that the ability to bind inulin was widespread in the microbiota. We further evaluate which taxa are metabolically stimulated by inulin and find that diverse taxa from the phyla Firmicutes and Actinobacteria respond to inulin, and several isolates of these taxa can degrade inulin. Incubation with another prebiotic, xylooligosaccharides (XOS), in contrast, shows a more robust bifidogenic effect. Interestingly, the Coriobacteriia Eggerthella lenta and Gordonibacter urolithinfaciens are indirectly stimulated by the inulin degradation process, expanding our knowledge of inulin-responsive bacteria.

Research Organization:
University of North Carolina, Chapel Hill, NC (United States)
Sponsoring Organization:
USDOE Office of Science (SC); European Research Council; Austrian Science Fund; Novo Nordisk Foundation
Grant/Contract Number:
SC0019012
OSTI ID:
2472302
Journal Information:
Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 14; ISSN 2041-1723
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
bacteria
inulin
microbiome diversity
prebiotics